NG Biotech

NG Biotech Antimicrobial Resistance (AMR) Testing

Ensuring appropriate use of tailored antibiotics and supporting #antimicrobialstewardship.
Una Health are committed to becoming an advocate for change in response to the global threat of AMR.

The range of tests from NG Biotech have been developed in collaboration with the French CEA and are a new range of rapid tests for Antimicrobial Resistance (AMR) detection from cultured colonies. These cutting-edge antimicrobial resistance tests enable rapid detection in just 15 minutes with results you can trust. The patented new multi-layer, multi conjugate lateral flow platform utilised across the NG AMR product range increases the capacity of key biomarkers, whilst maintaining the high quality of detection.

A range of rapid tests for detection of antimicrobial resistance mechanisms direct from bacterial cultures.

The NG-Test AMR platform is a visual, multiplex lateral flow immunochromatographic assay, developed using patented technology. Each single use cassette offers rapid, qualitative detection and differentiation of different antimicrobial resistance mechanisms among non-susceptible colonies of Gram-negative bacteria.

NG Biotech CARBA-5

NG-Test CARBA 5 aids in the rapid identification of carbapenemase-producing Enterobacterales and Pseudomonas aeruginosa in healthcare settings.

NG-Test CARBA-5 detects one or more of the five common types of carbapenemase enzymes (KPC (K), OXA-48-like (O), IMP (I), VIM (V), NDM (N)) from carbapenem-non-susceptible Gram-negative bacterial colonies.

NG Biotech CTX-M & CTX-M MULTI

The production of beta-lactamases, enzymes that degrade the beta-lactam ring of the beta-lactam family of antibiotics, are a common resistance mechanism found in Gram-negative bacteria, particularly Enterobacterales.

There are different classes of beta-lactamase, each of which has a different spectrum of activity. The CTX-M class of beta-lactamases is important due to the extended spectrum of activity against penicillins and cephalosporins, including third generation cephalosporins. This makes Gram-negative bacteria that carry CTX-M enzymes more difficult to treat, requiring broader spectrum agents, such as piperacillin/tazobactam.

The NG Biotech CTX-M & CTX-M MULTI tests are an important tool that identify one of the important mechanisms of resistance to cephalosporins commonly found in Enterobacterales and inform appropriate prescribing #antimicrobialstewardship.

NG-Test CTX-M detects the presence of variants belonging to group 1 CTX-M enzymes, including CTX-M-15, considered the most common in many global locations.

NG-Test CTX-M multiplex detects variants belonging to all five known CTX-M groups: 1, 2, 8, 9 & 25.

NG Biotech MCR-1

In terms of multidrug-resistant Gram-negative bacteria, colistin may be the only antibiotic to which the bacteria exhibit susceptibility, making it the last option available for treatment.

Colistin resistance is intrinsic in some bacterial species, such as Serratia and Proteus species. Resistance to colistin may arise due to chromosomal mutations; however, the advent of colistin resistance associated with a mobile genetic element (plasmid) is of serious concern.

The mcr gene, mobilised colistin resistance, encodes a phosphoethanolamine transferase that modifies the structure of the outer membrane of Gram-negative bacteria and decreases the activity of colistin against bacterial cells. The carriage of the mcr gene on mobile genetic elements means that there is a risk that colistin resistance will spread among other bacteria, including those that are resistant to all other classes of antibiotic.

The NG-Test MCR-1 test is an important tool that identifies an important mechanism of resistance to colistin to inform appropriate prescribing #antimicrobialstewardship and infection prevention actions.

Reliable results in 15 minutes
Detects multiple mechanisms
Supports #antimicrobialstewardship

How the NG-Test AMR test platform works

The NG-Test AMR platform is based on lateral flow immunochromatographic principles:

  • Monoclonal antibodies that individually recognize each of the antimicrobial degrading enzymes (carbapenemases OR extended spectrum β-lactamases OR phosphoethanolamine transferase) are immobilized onto a nitrocellulose strip.
  • Free (mobile) monoclonal antibodies are present in the conjugate pad and labelled with colloidal gold.
  • When bacterial colonies are mixed with the extraction buffer and added to the sample pad, the capillary action of the nitrocellulose draws the lysed bacterial cell suspension through the mobile labelled-antibodies on the conjugate pad.
  • If present in the suspension the target analyte will bind with the mobile labelled-antibodies and the molecular complex will move towards the immobile antibodies present on the test lines.
  • If the target is not present in the suspension, the mobile labelled-antibodies will move along the test strip.
  • If the target analyte is present in the suspension, the mobile labelled-antibodies+ target analyte molecular complex will be captured by the immobile antibodies present on the test line/s and a change in colour at the test line will occur.
  • If the target is not present in the suspension, the mobile labelled-antibodies will not be captured by the immobile antibodies at the test lines but will continue along the strip and be captured by the control line, causing a change in colour at the control line only.

How to interpret results

  • A positive result: a red line appears on the control region (C) and one or more lines appear in the test regions and indicates that the bacterial suspension contains the particular antimicrobial degrading enzyme under investigation.
  • A negative result: red line appears on the control region (C) with no lines in any of the test regions and indicates that the bacterial suspension does not contain the antimicrobial degrading enzyme under investigation.
  • If the control line does not appear, the test result is invalid.

How the NG-Test AMR test platform helps

Rapid detection of antimicrobial resistant bacteria is paramount to a patient receiving appropriate treatment in a timely manner and is necessary to inform infection prevention actions.

The NG Biotech range offers the following AMR tests:

  • CARBA-5 for rapid detection of carbapenemases
  • CTX-M and CTX-M Multi for rapid detection of the CTX-M family of extended spectrum beta-lactamases (ESBL)
  • MCR-1 for rapid detection of mobilised colistin resistance

The NG Biotech lateral flow kits offer equivalent results to molecular detection methods in a fraction of the time, with no need for additional equipment and associated maintenance costs. Hands on time to prepare the test is minimal and results are available to read in 15 minutes.

How to use the NG Biotech AMR Tests

The general procedure for all the NG AMR tests is similar:

NG How to use

How to interpret results

  • A positive result: a red line appears on the control region (C) and one or more lines appear in the test regions and indicates that the bacterial suspension contains the particular antimicrobial degrading enzyme under investigation.
  • A negative result: red line appears on the control region (C) with no lines in any of the test regions and indicates that the bacterial suspension does not contain the antimicrobial degrading enzyme under investigation.
  • If the control line does not appear, the test result is invalid.

Downloads

NG Carba-5 Technical Briefing

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